Publications

39. Park, H.B.*, Perez, C.E.*, Barber, K., Rinehart, J., Crawford, J.M.† 2017. Genome mining unearths a hybrid nonribosomal peptide synthetase-like-pteridine synthase biosynthetic gene cluster. eLife. 6: e25229. 

     See Yale West Campus News. Atherton, J. 2017. New molecules unearthed for future medical discovery. March 31.

38. Park, H.B., Sampathkumar, P., Perez, C.E., Lee, J.H., Tran, J., Bonanno, J.B., Hallem, E.A., Almo, S.C., Crawford, J.M.† 2017. Stilbene epoxidation and detoxification in a Photorhabdus luminescens-nematode symbiosis. J. Biol. Chem. 292(16): 6680-6694. 

37. Trautman, E.P., Healy, A.R., Shine, E.E., Herzon, S.B., Crawford, J.M.† 2017. Domain-targeted metabolomics delineates the heterocycle assembly steps of colibactin biosynthesis. J. Am. Chem. Soc. 139(11): 4195-4201.   

     Selected as JACS Spotlight. Lockwood, D. 2017. Drilling down into multidomain enzymes to image small-molecule synthesis. J. Am. Chem. Soc. 139(13): 4583. 

36. Torring, T., Shames, S.R., Cho, W., Roy, C.R., Crawford, J.M.† 2017. Acyl-histidines: New N-acyl-amides from Legionella pneumophila. ChemBioChem. 18(7): 638-646.

35. Fischer, C.N., Trautman, E.P., Crawford, J.M., Stabb, E.V., Handelsman, J., Broderick, N.A.† 2017. Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior. eLife. 6: e18855.  

34. Healy, A.R., Nikolayevskiy, H., Patel, J., Crawford, J.M., Herzon, S.B.† 2016.  A mechanistic model for colibactin-induced genotoxicity. J. AM. Chem. Soc. 138 (48): 15563-15570.

33. Park, H.B., Perez, C.E., Perry, E.K., Crawford, J.M.† 2016. Activating and attenuating the amicoumacin antibiotics. Molecules. 21(7): 824. 

32. Park, H.B., Crawford, J.M.† 2016. Pyrazinone protease inhibitor metabolites from Photorhabdus luminescens. J. Antibiot. 69(8): 616-621.  

31. Healy, A.R., Vizcaino, M.I., Crawford, J.M., Herzon, S.B. 2016. Convergent and modular synthesis of candidate precolibactins. Structural revision of precolibactin A. J. Am. Chem. Soc. 138(16): 5426-5432. 

30.​ Trautman, E., Crawford, J.M.† 2016. Linking biosynthetic gene clusters to their metabolites via pathway-targeted molecular networking. Curr. Topic. Med. Chem. 16(15): 1705-1716.

29. Vizcaino, M.I., Crawford, J.M.† 2016. Secondary metabolic pathway-targeted metabolomicsMeth. Mol. Biol. 1401: 175-195.  

28. Park, H.B., Crawford, J.M.† 2015. Lumiquinone A: An alpha-aminomalonate-derived aminobenzoquinone from Photorhabdus luminescens. J. Nat. Prod. 78(6): 1437-41. 

27. Vizcaino, M.I., Crawford, J.M.† 2015. The colibactin warhead crosslinks DNA. Nature Chem.7: 411-7. 

     News & Views: Schmidt, E. 2015. Natural Products: Hunting microbial metabolites. Nature Chem. 7:375-6.  F1000: Tang, G.-L. 2015. Excellent Recommendation. Research in the News: Shelton, J. 2015. Spotting a molecular warhead for disease in the human gut. YaleNews. April 6.

26. Engel, P.,† Vizcaino, M.I., Crawford, J.M. 2014. Gut symbionts from distinct hosts exhibit genotoxic activity via divergent colibactin biosynthetic pathways. Appl. Environ. Microbiol.81(4): 1502-12. 

25. Guo, X., Crawford, J.M.† 2014. An atypical carbohydrate-NRPS genomic island encodes a novel lytic transglycosylase. Chem. Biol. 21(10): 1271-7. 

     Research in the News: Shelton, J. 2014. Setting a course for genomic islands. YaleNews. September 15. 

24. Vizcaino, M.I., Engel, P., Trautman, E., Crawford, J.M.† 2014. Comparative metabolomics and structural characterizations illuminate colibactin pathway-dependent small molecules. J. Am. Chem. Soc. 136(26): 9244-7. 

23. Vizcaino, M.I., Guo, X., Crawford, J.M.† 2014. Merging chemical ecology with bacterial genome mining for secondary metabolite discovery. J. Ind. Microbiol. Biotechnol. 41(2): 285-99.

     Special Issue: Invited manuscript to be included in the Special Issue on Microbial Genome Mining dedicated to Sir David Hopwood on the occasion of his 80th birthday. Nature’s Drugs: Allen, J. 2013. The search for organic treatment products. Yale Scientific Magazine. December.

22. Vagstad, A.L., Newman, A.G., Storm, P.A., Belecki, K., Crawford, J.M., Townsend, C.A.† 2013. Combinatorial Domain Swaps Provide Insights into the Rules of Fungal Polyketide Synthase Programming and the Rational Synthesis of Non-Native Aromatic Products. Angew. Chem. Int. Ed.52(6): 1718-21. 

21. Somvanshi, V.S., Sloup, R.E., Crawford, J.M., Martin, A.R., Heidt, A.J., Kim, K.-S., Clardy, J., Ciche, T.A.† 2012. A Single Promoter Inversion Switches Photorhabdus Between Pathogenic and Mutualistic States. Science. 337: 88-93. 

     Research Highlights: David, R. 2012. Bacterial pathogenesis: The two faces of P. luminescens. Nature Rev. Micro. 10 (9): 594. News: Yong, E. 2012. Worm kills insects by vomiting Hulk-like bacteria. Discover. July 5. News: Noonan, T. 2012. Gene switch that turns bacteria into mighty Hulk. NewScientist. July. News: Cameron, L. 2012. Jekyll and Hyde bacteria aids or kills, depending on chance. Michigan State University News and in ScienceDaily. July 5.

20. Crawford, J.M.*, Portmann, C.*, Zhang, X., Roeffaers, M.B.J., and Clardy, J.† 2012. Small molecule perimeter defense in entomopathogenic bacteria. Proc. Natl. Acad. Sci. USA. 109 (27): 10821-10826. 

     Nature Research Highlights: 2012. Microbiology: Bacterial border defence. Nature. 486: 297.

19. Crawford, J.M. and Clardy, J.† 2012. Microbial genome mining answers longstanding biosynthetic questions. Proc. Natl. Acad. Sci. USA. 109 (20): 7589-90. 

18. Crawford, J.M., Portmann, C., Kontnik, R. Walsh, C.T., and Clardy, J.† 2011. NRPS substrate promiscuity diversifies the xenematides. Org. Lett. 13 (19): 5144-5147. 

17. Crawford, J.M.*, Mahlstedt, S.A.*, Malcolmson, S.J., Clardy, J., and Walsh, C.T.† 2011. Dihydrophenylalanine: A prephenate-derived Photorhabdus luminescens antibiotic and intermediate in dihydrostilbene biosynthesis. Chem. Biol. 18: 1102-1112. 

16. Crawford, J.M. and Clardy, J.† 2011. Bacterial symbionts and natural products. Chem. Commun. 47: 7559-7566.

15. Crawford, J.M. and Townsend, C.A.† 2010. New insights into the formation of fungal aromatic polyketides. Nature Rev. Micro. 8 (12): 879-889. 

14. Crawford, J.M. and Clardy, J.† 2010. Biosynthesis: Not just passing through. Nature Chem.2: 805-807.

13. Kontnik, R.*, Crawford, J.M.*, and Clardy, J.† 2010. Exploiting a global regulator for small molecule discovery in Photorhabdus luminescens. ACS Chem. Biol. 5 (7): 659-665. [selected for cover; Maximizing Metabolites] 

     2010. Introducing Our Authors. ACS Chem. Biol. 5 (7): 626-627.

12. D’Onofrio, A.*, Crawford, J.M.*, Stewart, E.J., Witt, K. Gavrish, E. Epstein, S., Clardy, J.†, and Lewis, K.† 2010. Siderophores from neighboring organisms promote the growth of uncultured bacteria. Chem. Biol. 17: 254-264. [selected for cover; Culturing the Uncultured]

     The Loom: Zimmer, Carl. 2010. Luring out the missing biosphere. Discover. March 26. Hidden Jewels: Schipani, V. 2010. Bacteria need friendly neighbors. Top 7 Hidden Jewels (#2), Faculty of 1000. October 26. Feature: Potera, C. 2010. Siderophores shed light on the “great plate count anomaly.” Microbe. Feature and podcast. August. ASM Blog: Lewis, K. Small things considered: The uncultured bacteria. The Microbe Blog. July 12.

11. Ehrlich, K.C.*†, Chang, P.-K.*, Scharfenstein, L., Cary, J.W., Crawford, J.M., and Townsend, C.A. 2010. Absence of the aflatoxin biosynthesis gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures. FEMS Microbiol. Lett. 305: 65-70. 

10. Korman, T.P.*, Crawford, J.M.*, Labonte, J., Wong, J. Townsend, C.A.†, and Tsai, S.-C.† 2010. Structure and function of an iterative polyketide synthase thioesterase domain catalyzing Claisen cyclization in aflatoxin biosynthesis. Proc. Natl. Acad. Sci. USA. 107: 6246-6251.

9. Crawford, J.M.*, Kontnik, R.*, and Clardy, J.† 2010. Regulating alternative lifestyles in entomopathogenic bacteria. Current Biology. 20: 69-74. [selected for cover; Signaling in a Trilateral Symbiosis]

     Dispatch: Waterfield, N. 2010. Host-pathogen interactions: Proline gives insect pathogens the green light. Current Biology. 20: R13-15. Focus: Garces, R. and Neal, R. 2010. Jekyll-and-Hyde switch may turn off infection. Harvard Focus. February 5.  Crimson News: Stone, J.L. 2010. Roundworm bacteria research shows promise for development of new antibiotics. The Harvard Crimson. February 19. Editor’s Choice: Gough, N.R. 2010. Host-pathogen interactions: Proline promotes virulence. Sci. Signal. 3(106): ec31.

8. Crawford, J.M*, Korman, T.P.*, Labonte, J.W., Vagstad, A.L., Hill, E.A., Kamari-Bidkorpeh, O., Tsai, S.-C.†, and Townsend, C.A.† 2009. Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization. Nature. 461: 1139-1143.

     News & Views: Sherman, D.H. Enzyme’s black box cracked open. 2009. Nature. 461: 1068-1069. Research Highlights: Molloy, S. Fungal metabolism: Completing the circle. 2009. Nature Rev. Micro. 7: 842. Highlight: Weissman, K.J. Peering into the black box of fungal polyketide biosynthesis. 2010. ChemBioChem. 11: 485-488. Science Highlights: Hanna, R. Deadly carcinogen unraveled: the molecular origami of fungal polyketides. 2010. SSRL Science Highlights. March 31.

7. Belecki, K., Crawford, J.M., and Townsend, C.A.† 2009. Production of octaketide polyenes by the calicheamicin polyketide synthase CalE8: implications for the biosynthesis of enediyne core structures. J. Am. Chem. Soc. 131: 12564-12566.

6. Crawford, J.M., Vagstad, A.L., Ehrlich, K.C., Udwary, D.W., and Townsend, C.A.† 2008. Acyl-carrier protein - phosphopantetheinyltransferase partnerships in fungal fatty acid synthases. ChemBioChem. 9: 1559-1563.

5. Crawford, J.M., Vagstad, A.L., Ehrlich, K.C., and Townsend, C.A.† 2008. Starter unit specificity directs genome mining of polyketide synthase pathways in fungi. Bioorg. Chem. 36: 16-22.4.

4. Crawford, J.M., Vagstad, A.L., Whitworth, K.P., Ehrlich, K.C., and Townsend, C.A.† 2008. Synthetic strategy of nonreducing iterative polyketide synthases and the origin of the classical “starter unit effect.” ChemBioChem. 9: 1019-1023.

3. Crawford, J.M., Thomas, P.M., Scheerer, J.R. Vagstad, A.L., Kelleher, N.L.†, and Townsend, C.A.† 2008. Deconstruction of iterative multidomain polyketide synthase function. Science. 320: 243-246.

     Perspective: Weissman, K.J. Anatomy of a fungal polyketide synthase. 2008. Science. 320:186-187. Research Highlights: Smallridge, R. Fungal metabolism: Dissecting catalysis.2008. Nature Rev. Micro. 6: 414-415. Concentrates: Fungal enzyme deconstructed. Chemical & Engineering News. 2008. 86: 50-51.

2. Townsend, C.A.†, Crawford, J.M., and Bililign, T. 2006. New images evoke FAScinating questions. Chem. Biol. 4: 349-351.

1. Crawford, J.M., Dancy, B.C.E., Hill, E.A., Udwary, D.W., and Townsend, C.A.† 2006. Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase. Proc. Natl. Acad. Sci. USA. 103: 16728-16733.